A technique for identifying molecules present on the surfaces of cells or in tissues using a highly fluorescent substance coupled to a specific antibody.
A laboratory procedure using fluorescein- labeled antibodies that aid in detecting antigens and the diagnosis of infectious diseases.
A technique for observing the amount and/or distribution of antibody or antigen in a tissue section. The antibodies are labeled (directly or indirectly) with a fluorescent dye (e.g. fluorescein) and applied to the tissue, which is observed through an ultraviolet microscope. In direct immunofluorescence the antibody is labeled before being applied to the tissue. In indirect immunofluorescence the antibody is labelled after it has bound to the antigen, by means of fluorescein-labeled anti-immunoglobulin serum.
The detection of antibodies with fluorescein-labeled proteins. Bound antibodies glow when illuminated.