Protein engineering

The selective, deliberate (re)designing and synthesis of proteins. This is done in order to cause the resultant proteins to carry out desired (new) functions. Protein engineering is accomplished by changing or interchanging individual amino acids in a normal protein. This may be done via chemical synthesis or recombinant DNA technology (i.e., genetic engineering). “Protein engineers” (actually genetic engineers) use recombinant DNA technology to alter a particular nucleoside or triplet (codon) in the DNA (genes) of a cell. In this way it is hoped that the resulting DNA codes for the different (new) amino acid in the desired location in the protein produced by that cell.


 


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