Autoradiography

Photographic recording of radiation from radioactive material obtained by placing the surface of the radioactive material in proximity to a detector sensitive to the emitted spectrum, most commonly X‐ray film or a charge coupled device.


A technique to detect radioactively labeled molecules by creating an image on photographic film. The slab of gel or other material in which the molecules are held (suspended) is placed on top of a piece of photographic film. The two are then securely fastened together such that movement is eliminated and the film is exposed for a period of time. The exposed (to the radiation) film is subsequently developed and the radioactive area is seen as a dark (black) area. Among other uses, autoradiography has been used to track the spread of (radioactively labeled) viruses in a living plant. After treatment (i.e., the radioactive labeling process), the whole plant (in a slab) is placed on top of a piece of photographic film. When the film is subsequently developed, the “picture” seen is of a plant, with darker areas indicating regions of greater virus concentration.


A record or photograph prepared by labeling a substance with radioactive material and allowing the image to develop on a film over a period of time.


Detection of radioactive molecules by visualization on photographic film.


A technique for examining the distribution of a radioactive tracer in the tissues of an experimental animal. The tracer is injected into the animal, which is killed after a certain period. Thin sections of its organs are placed in close contact with a radiation sensitive material, such as a photographic emulsion, and observed under a microscope. Blackening of the film indicates a high concentration of radioactive material.


The radiograph formed by radioactive materials present in the tissue or individual. This is made possible by injecting radio chemicals into the person or tissue and then exposing x-ray film by placing the individual or tissue adjacent to the film.


Use of autoradiographs in investigating certain diseases.


The measurement of radiation emitted by radioactive material in an object is obtained by placing the object in close proximity to a photosensitive emulsion, resulting in a recorded image.


 


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