Category: H
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Hot air sterilization
Used for glass, metal and paper articles in the laboratory, and for powders, fats and oils pharmaceutically. Penetration of hot air is slow, and a fan is necessary to maintain an even temperature in all parts of the load. The process is divided into three periods: (1) heating up period (about 1 hour for an…
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Holotype
The original culture of a described bacteriological species (deposited in a national collection of type cultures).
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Heterophile antibodies
Antibodies reacting with an antigen which has no biological relationship to the immunizing stimulus.
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Hetacillin
Semi-synthetic penicillin possessing very similar properties to ampicillin, to which it hydrolyses in solution and in vivo.
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Hemming’s filter
Micro-filter consisting of a metal holder for a membrane or asbestos pad filter, with a 5 cm³ (bijou) vital screwed at each end. The assembled apparatus is centrifuged, forcing fluid from one vial through the filter into the receptor vial.
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Helber counting chamber
Cell counting chamber for the enumeration of bacteria, consisting of a thick microscope slide with a depressed platform 0.02 mm lower than the top surface, ruled with Thoma ruling—i.e., a 1 mm square subdivided into 400 squares each 1/20 x 1/20 mm (0.0025 mm² area). With the special coverslip in position 0.02 mm above this…
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Heiberg classification
A classification of the cholera vibrios into 6 groups by their production of acid from mannose, sucrose and arabinose. Group 1 (mannose and sucrose positive) contains the classical pathogenic vibrios.
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Hanging drop
Microscopical examination of bacteria—especially for motility—by observing the organisms in a drop of culture fluid hanging from the underside of a coverslip. The drop is kept from contact with the carrying slide by a cavity in the thickness of the latter, or by separating a plain slide from the coverslip by small portions of plasticene.…
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Hafnia
Genus within the family of the Enterobacteriaceae. Motile, non- pathogenic and non-lactose-fermenting, they produce consistent biochemical reactions only when incubated at 20°C, results at 37°C being unpredictable. Urease negative, KCN positive.
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Haffkine’s vaccine
Prophylactic vaccine for protection against Pasteurella pestis, a heat-killed culture of that organism.