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Nutrient broth with high glucose and serum levels, used as a protective medium for the suspension of delicate bacteria preparatory to freeze-drying.  

Bacteriological nutrient broth plus glucose, acting as both nutrient and reducing agent, usually at 0.1 to 1.0 per cent.  

Nutrient agar plus 0.1 -1.0 per cent glucose, used for stab cultures of anaerobic organisms.  

Characterization test for coliform organisms, a positive result showing ability of the culture to oxidize potassium gluconate to 2 keto- gluconate. All Enterobacter species are positive; only a few others exhibit the trait.  

Gelatin was used to solidify culture media prior to the advent of agar-agar. Its property of liquefying at around 28°C limited the possibilities of use.  

The possession of proteolytic properties is an important secondary characteristic of many species of bacteria (especially enterobacteria and Clostridia) and is determined by the ability of the culture to hydrolyse gelatin. In the traditional method a nutrient gelatin butt is inoculated and incubated, hydrolysis being indicated by liquefaction of the medium. Up to 14 days…

Methods for the assay or identification of antigens or antibodies in bacterial species. Interacting antigen and antibody diffuse toward each other through agar or gelatin, any resultant precipitation being seen as an opaque line at the point of contact. Secondary lines may appear, denoting further antigens or antibodies. An application of the method is Elek’s…

Plastic envelope which, with the addition of water, evolves hydrogen for use in an anaerobic jar with cold catalyst.