Mindblown: a blog about philosophy.
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Enterobacter aerogenes
Second species in the genus, formerly known as Aerobacter aerogenes. Ferments inositol and adonitol. A species that occurs normally in the intestines of humans and other animals and is found in decayed matter, on grains, and in plants. It causes opportunistic infections of the urinary tract and of the intestine when antibiotic therapy diminishes other…
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Enrichment media
Bacteriological culture media designed to encourage growth of a selected group of bacteria (e.g.. Salmonella) while inhibiting unwanted organisms (e.g., Esch. coli) in a mixed inoculum such as faeces. Enrichment media are usually fluid. After incubation the enriched culture is sub-cultivated on solid medium.
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Ellner’s medium
Buffered yeast-extract peptone water medium for inducing spore formation in Cl. welchii.
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Elek’s medium
Buffered urea solution which is Nesslerized after addition of a grown culture of enterobacteria, urease activity being indicated by a yellow colour.
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Dysgonic
Literally ‘difficult seeding’; bacteriologically the term implies cultivation which is slow and difficult, and it is used especially of some mycobacteria.
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Dubos medium
Synthetic liquid medium for the cultivation of tubercle bacilli. Two varieties are used: (1) with Tween 80, for dispersed growth of acid- fast bacilli; (2) with oleic acid, to aid the production of ‘cords’ of virulent growing bacteria.
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Dropping pipettes
Pasteur pipettes designed to deliver drops of designated size (25/cm³, 50/cm³, etc) in serology tests, plate counts and other methods. The drop size is determined by the external diameter of the pipette tip, and appropriate sizes are obtained by the use of a Starrett Standard Wire Gauge (0.95 mm) hole of the gauge and cut…
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Diphtheria virulence test
Strains of known or suspected C. diphtheriae are tested for virulence by their intramuscular injection into 2 guinea-pigs, one of which is first immunized with diphtheria antitoxin. If the strain is virulent, the non-immunized animal will die within 3 days.
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Digest media
Bacteriological nutrient broths (and agars prepared from them) in which the digested protein is not added as peptone, but is derived from the action of enzymes such as trypsin digesting partially cooked meat in an alkaline state at 37°C. In Hartley’s broth the digestant is an alcoholic extract of porcine pancreas (Cole and Onslow’s pancreatic…
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Differential media
Culture medium allowing easy visualization of colonial differences between two types of bacteria—e.g., the red colonies of lactose- fermenting conforms on MacConkey agar as opposed to the colourless colonies of S. typhi
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