Salting out

A technique used for forcing (dissolved) proteins out of a solution by increasing the concentration of salt in the solution. The Na+ and CI- ions derived from the salt compete for and “tie up” water molecules that are solubilizing the protein molecules thereby rendering them insoluble or more insoluble.


A method of separating a specific protein from a mixture of proteins by the addition of a salt (e.g., ammonium sulfate).


 


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