{"version":"1.0","provider_name":"Glossary","provider_url":"https:\/\/www.healthbenefitstimes.com\/glossary","author_name":"Glossary","author_url":"https:\/\/www.healthbenefitstimes.com\/glossary\/author\/adminglossary\/","title":"Ligase chain reaction - Definition of Ligase chain reaction","type":"rich","width":600,"height":338,"html":"<blockquote class=\"wp-embedded-content\" data-secret=\"SazOGSwNia\"><a href=\"https:\/\/www.healthbenefitstimes.com\/glossary\/ligase-chain-reaction\/\">Ligase chain reaction<\/a><\/blockquote><iframe sandbox=\"allow-scripts\" security=\"restricted\" src=\"https:\/\/www.healthbenefitstimes.com\/glossary\/ligase-chain-reaction\/embed\/#?secret=SazOGSwNia\" width=\"600\" height=\"338\" title=\"&#8220;Ligase chain reaction&#8221; &#8212; Glossary\" data-secret=\"SazOGSwNia\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" class=\"wp-embedded-content\"><\/iframe><script>\n\/*! This file is auto-generated *\/\n!function(d,l){\"use strict\";l.querySelector&&d.addEventListener&&\"undefined\"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!\/[^a-zA-Z0-9]\/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret=\"'+t.secret+'\"]'),o=l.querySelectorAll('blockquote[data-secret=\"'+t.secret+'\"]'),c=new RegExp(\"^https?:$\",\"i\"),i=0;i<o.length;i++)o[i].style.display=\"none\";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute(\"style\"),\"height\"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):\"link\"===t.message&&(r=new URL(s.getAttribute(\"src\")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener(\"message\",d.wp.receiveEmbedMessage,!1),l.addEventListener(\"DOMContentLoaded\",function(){for(var e,t,s=l.querySelectorAll(\"iframe.wp-embedded-content\"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute(\"data-secret\"))||(t=Math.random().toString(36).substring(2,12),e.src+=\"#?secret=\"+t,e.setAttribute(\"data-secret\",t)),e.contentWindow.postMessage({message:\"ready\",secret:t},\"*\")},!1)))}(window,document);\n\/\/# sourceURL=https:\/\/www.healthbenefitstimes.com\/glossary\/wp-includes\/js\/wp-embed.min.js\n<\/script>\n","description":"A technique for amplifying the quantity of specific sequences of nucleic acid in a specimen. The patient\u2019s DNA, or specimens thought to contain pathogenic DNA, are mixed with DNA ligase and oligonucleotide probes. Double-stranded DNA is denatured. Probes bind to the complementary strands on any denatured target DNA. Ligase joins the bound probes, and multiple copies of the DNA of interest are made. In clinical practice, ligase chain reactions are used primarily in urinary (noninvasive) assays to detect genital infections with chlamydia or gonorrhea.\u00a0"}