A very sensitive method of quantitating a specific antigen using a specific radiolabeled antibody. Functionally, the antibody is made radioactive by the covalent incorporation of radioactive iodine. The radioimmuno probe thus prepared is exposed to its antigen (which may be a protein, or a receptor, etc.) in excess (the exact amount will have to be determined). The radiolabeled probe then binds to the antigen and the unbound, free probe is washed away. The radioactivity is then determined (counted) and by comparison to a standard plot which has been constructed previously, the amount of antigen (binding) is determined.
Test that measures minute amounts of antibodies or antigens by the use of radioactive substances.
A test whereby the blood of a woman is analyzed to determine whether pregnancy exists.
The use of radioactive tracers to investigate the presence of antibodies in blood samples, in order to measure the antibodies themselves or the amount of particular substances, such as hormones, in the blood.
Method of determining the concentration of a protein in the serum by monitoring any reaction produced by the injection of a radioactively labeled substance known to react in a particular way with the protein being studied.
Study of antigens, antibodies, enzymes, and hormones using radiolabeled reactants.
A method of determining the concentration of a substance, especially hormones, based on the competitive inhibition of binding of a radioactively labeled substance to a specific antibody. Protein concentrations in the picogram (10‾¹² g) range can be measured by this technique.
A precise laboratory method employing radioactive isotopes to gauge the concentration of particular proteins, like hormones or antibodies, within an individual’s bloodstream.