A sterilization process carried out on three consecutive days by exposure to moist steam at 100°C.
Sterilization of fluids (culture media especially) by 3 daily exposures to free steam at 100°C for 20-45 minutes. The method is reserved for media (e.g., carbohydrate) liable to decomposition at higher temperatures. All vegetative bacteria are killed by the first steaming; such spore bearers as survive then become vegetative between steamings and are killed by the subsequent heating. The method may not successfully sterilize thermophiles or anaerobic spore bearers.